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mouse embryonic fibroblast cells  (ATCC)
99
ATCC mouse embryonic fibroblast cells
(A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic <t>fibroblast</t> (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.
Mouse Embryonic Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse embryonic fibroblast cells - by Bioz Stars, 2026-02
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cf 1 mouse embryonic fibroblast mef feeder cells  (AMS Biotechnology)
96
AMS Biotechnology cf 1 mouse embryonic fibroblast mef feeder cells
(A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic <t>fibroblast</t> (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.
Cf 1 Mouse Embryonic Fibroblast Mef Feeder Cells, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cf 1 mouse embryonic fibroblast mef feeder cells/product/AMS Biotechnology
Average 96 stars, based on 1 article reviews
cf 1 mouse embryonic fibroblast mef feeder cells - by Bioz Stars, 2026-02
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mouse embryonic fibroblast mef cells  (ATCC)
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ATCC mouse embryonic fibroblast mef cells
(A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic <t>fibroblast</t> (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.
Mouse Embryonic Fibroblast Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse embryonic fibroblast mef cells/product/ATCC
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mouse embryonic fibroblast mef cell line nih 3t3  (ATCC)
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ATCC mouse embryonic fibroblast mef cell line nih 3t3
(A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic <t>fibroblast</t> (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.
Mouse Embryonic Fibroblast Mef Cell Line Nih 3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse embryonic fibroblast mef cell line nih 3t3/product/ATCC
Average 99 stars, based on 1 article reviews
mouse embryonic fibroblast mef cell line nih 3t3 - by Bioz Stars, 2026-02
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cell lines human embryonic kidney 293t atcc crl 3216 mouse embryonic fibroblasts mefs  (ATCC)
99
ATCC cell lines human embryonic kidney 293t atcc crl 3216 mouse embryonic fibroblasts mefs
(A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic <t>fibroblast</t> (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.
Cell Lines Human Embryonic Kidney 293t Atcc Crl 3216 Mouse Embryonic Fibroblasts Mefs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cell lines human embryonic kidney 293t atcc crl 3216 mouse embryonic fibroblasts mefs/product/ATCC
Average 99 stars, based on 1 article reviews
cell lines human embryonic kidney 293t atcc crl 3216 mouse embryonic fibroblasts mefs - by Bioz Stars, 2026-02
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mouse embryonic fibroblast mef cell lines from423 atcc  (ATCC)
97
ATCC mouse embryonic fibroblast mef cell lines from423 atcc
(A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic <t>fibroblast</t> (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.
Mouse Embryonic Fibroblast Mef Cell Lines From423 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse embryonic fibroblast mef cell lines from423 atcc/product/ATCC
Average 97 stars, based on 1 article reviews
mouse embryonic fibroblast mef cell lines from423 atcc - by Bioz Stars, 2026-02
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(A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic fibroblast (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.

Journal: PLOS Pathogens

Article Title: Nucleus softens during herpesvirus infection

doi: 10.1371/journal.ppat.1013873

Figure Lengend Snippet: (A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic fibroblast (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.

Article Snippet: Mouse embryonic fibroblast cells (MEF, ATCC CRL-2991) and African green monkey kidney cells (Vero, ATCC CCL-81) were grown in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum, L-glutamine, and penicillin-streptomycin (Gibco-Invitrogen) at 37 °C in the presence of 5% CO 2.

Techniques: Tomography, Infection, Electron Microscopy, Standard Deviation, Labeling